Pole tekstowe: Dr Dariusz Kaczmarczyk Fotka

 

Curriculum Vitae

09 19 1978    Born in Olsztyn (Northern East Poland)

1997               Graduation at at III Lyceum in Olsztyn University of Warmia and Mazury Entrance Qualification 

1997 – 2002  Study of Faculty of Environmental Sciences and Fisheries at the University of Warmia and Mazury in Olsztyn

2000 – 2002  Study on specialty of Biotechnology in Animal Breeding

2002               M.Sc. Faculty of Environmental Sciences and Fisheries

 University of Warmia and Mazury in Olsztyn

                         Thesis Title: Estimation of grownth rate of vendace (Coregonus albula) from lake Wigry

2006                 Ph.D. Faculty of Environmental Sciences and Fisheries

                          University of Warmia and Mazury in Olsztyn

  Domain: agricultural sciences
              Discipline: fishery

Dissertation Title: Evaluation of genetic polymorphism of in selected broodstocks of paddlefish (Polyodon spathula Walbaum 1849) based on microsatellite DNA sequences

2007-present   Employed in Department of Environmental Biotechnology

 

Teaching activity

 

genetic, ecotoxycology, and some other subjects including aquaculture, molecular and populaton genetic topics

 

Research Interests

Aplication of the microsatellite markers in:

1) Estimation of genetic variations within domesticated stock of commercial important species (paddlefish), endangered populations of salmonids, sturgeons and small cyprinid species (lake minnow) and genetic distance between them

2)  Management of the genetic variation resources within commercial stocks and natural populations

3) Enrichment of the genetic variation, and preventing of the inbreeding events within commercial stocks by assemblage pairs of spawners pairs following to their individual genetic differences (microsatellite loci)

4. The verivication of gynogenesis and androgenesis results applying sets of  microsatellite markers 

 

The list of most important methods and techniques that I apply during my studies

 

Field based techniques:

  • Fish marking (microchip markers)
  • Tissue sampling

Molecular techniques:

  • Extraction of DNA from various tissues (fragments of fins, skin, muscles)
  • Amplification of microsatellite loci using PCR technique (primers designing, optimisation of reaction parameters)
  • Assemblage of primers sets for multiplex PCR reactions  
  • Measurement of DNA fragments length using automated capillary DNA sequencers (Beckmann Coulter, Applied Biosystems)
  • Genotyping of microssatelite loci using  automatic capillary sequencers

Mathematical and statistical methods:

  • Estimation of genetic variation within populations and differences between them, evaluating of bottleneck events in population’s history,
  • Establishing of individual genetic profiles of fish,
  • Arrangement pairs of spawners in order to increase the heterozygosity of their progeny

 

Other

            2012- present member of editorial board of the journal Environmental Biotechnology.

 

Honors and Awards

 

2008. Award by Rector of Warmia and Mazury for an outstanding PhD thesis

2008 Award by Rector of Warmia and Mazury for achievement  in science

2008. Award by European Aquaculture society for Best Student Poster during Aquaculture Europe 2008 conference.

2017 2nd degree individual award for scientific achievements, granted by the Rector of the University of Warmia and Mazury in Olsztyn.

 

 


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