My research interests

    Most of the biological systems are organized in cells, witch may exist as unicellular or multicellular organisms. The cells are separated from their environment and from other cells by membranes. Because of the existence of the membranes each cell is a closed system. The biomembrane have a large variety of functions, and this is reflected in their structure and composition.

The fluid mosaic model of
biological membrane
Biomembranes are composed of lipids, proteins, carbohydrates and water in variable proportion. The commonly accepted model of membrane is the fluid mosaic model. More information you can find on page: http://anatomy.utmb.edu/cellbio/membrane.htm

I'a interesting in investigation of phisical properties of bilayer lipid membranes (BLM):

electrical properties
electroporation
transport through the membranes
development of measurement methods
mechanisms of BLM formation process
electrochemical sensors, biosensors based od lipid membrane


write to me!! were is BLM?
During experiments
The device for electrical measurements
The vessel used for 
formation of BLM (inside 
the Faraday case)

 

The vessel used for bilayer lipid membrane 
formation
I perform experiments using planar bilayer lipid membrane (BLM) prepared according to the Muller-Rudin method*. The membrane are formed in a vessel made from one pice of Teflon, consisting of two chembers. Between the chambers there is a septum with the aperture of about 1mm diameter. The formation of BLM is a spontaneus, self-assembling process.

* Mueller P., Rudin D., Tien H.T., and Wescott W.C., Reconstitution of excitable cell membrane structure in vitro, Circulation, 26 (1962) 1167-1171.


A photo of membrane made in a transmitted light
 Membrane formed from phosphatidylcholene dissolved in n-decane (20mg/ml); electrolyte 0.1M KCl, 0.01M HEPES, pH=7.0.


stankor@uwm.edu.pl