Most of the biological systems are organized in cells, witch may exist as unicellular or multicellular organisms. The cells are separated from their environment and from other cells by membranes. Because of the existence of the membranes each cell is a closed system. The biomembrane have a large variety of functions, and this is reflected in their structure and composition.
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electrical properties
electroporation
transport through the membranes
development of measurement methods
mechanisms of BLM formation process
electrochemical sensors, biosensors based od lipid
membrane
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formation of BLM (inside the Faraday case) |
The vessel used for bilayer lipid membrane formation |
I perform experiments using planar bilayer lipid membrane (BLM) prepared
according to the Muller-Rudin method*. The membrane are formed in a vessel
made from one pice of Teflon, consisting of two chembers. Between the chambers
there is a septum with the aperture of about 1mm diameter. The formation of
BLM is a spontaneus, self-assembling process.
* Mueller P., Rudin D., Tien H.T., and Wescott W.C., Reconstitution of excitable cell membrane structure in vitro, Circulation, 26 (1962) 1167-1171. |
A photo of membrane made in a transmitted light |
Membrane formed from phosphatidylcholene dissolved in n-decane (20mg/ml); electrolyte 0.1M KCl, 0.01M HEPES, pH=7.0. |