Kalinowski S., Ibron I., Bryl K., Figaszewski Z., . Biochim. Biophys. Acta., 1369 (1998) 204-212.

   The constant-intensity current chronopotentiometric measurements of egg yolk phosphatidylcholine bilayer membranes (BLM) are presented. It is demonstrates that a constant-intensity current flowing through the bilayer membranes generates the pores in their structures. For the current intensity from 0.1 to 2.0 nA, the generates pores open and close cyclically. The frequency of oscillations depends on the current intensity: the higher current intensity, the higher frequency of pore oscillations. It is suggested that the presented method may allow to create one pore in BLM and to observe its dynamical behavior. Based on chronopotentiometric curves, a method of pore conductance calculations is presented. It is demonstrated that the value of obtained conductance can be applied for pore diameter estimation. The hypothetical application of constant-intensity method as a biotechnological tool for selective and controlled incorporation of molecules into microorganisms is discussed.
 

2. Kotulska M., Koronkiewicz S., Kalinowski S., Cholesterol induced changes in the characteristics of the time series from planar lipid bilayer membrane during electroporation. Acta Phys. Pol., 33 (2002) 1115-1129.

3. Koronkiewicz S., Kalinowski S., Bryl K., Changes of structural and dynamic properties of model lipid membranes induced by a-tocopherol: implication to the membrane stabilization under external electric field. Biochim. Biophys. Acta., 1510 (2001) 300-306.

4. Koronkiewicz S., Bryl K., Cholesterol-induced variations in fluctuation of the pore in bilayer lipid membrane. Cell. Mol. Biol. Lett., 4 (1999) 567-582.

5. Koronkiewicz S., Bryl K., Witkowski S., Wałejko P., Changes of structural and dynamic properties of model lipid membranes induced by tocopherols. Natur. Sc., 3 (1999) 19-33.