This is my  M.Sc. thesis: ”Studies of the magnesium speciation in biological material using ultrafiltration and Atomic Absorption Spectroscopy (AAS) with atomisation in graphite furnace”. Supervisor: Prof. Adam Hulanicki (Warsaw University, Bialystok Branch, Department of Chemistry, Bialystok, Poland).

In medical science two different definitions of speciation coexist:
1. Chemical speciation - identification and quantification of the biologically active compounds to which the element is bound and the quantification of the element in relation to those particular molecules.
2. Speciation in cytology - the search for elements distribution in the cell.

Speciation of trace elements in body fluids facilitates defining the biocomponents to which the trace elements are bound and explains their mobility, storage, retention and toxicity. This is very important for understanding the essencial role of a many (not only trace) elements in human body. Investigation of speciations needs an interdisciplinary approach and is not easy. All analytical measurements should be accurate and precise, but results are offten very interesting and really exiting!!